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KMID : 1059519920360060864
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Journal of the Korean Chemical Society
1992 Volume.36 No. 6 p.864 ~ p.871
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Response Characteristics of pH-ISFET Urea Sensor
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Lee Heung-Lark
Yang Seung-Tae
Yun Jong-Hoon
Kim Chang-Soo
Sohn Byung-Ki
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Abstract
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A preparation method and response characteristics of a urea sensor which consisted of pH-ISFET and urease-immobilized membrane were investigated. The pH-ISFET urea sensor was fabricated by immobilizing BSA and urease with glutaraldehyde on gate of the pH-ISFET. Effects of pH and concentration of working buffer and enzyme load on the pontentiometric response of the pH-ISFET urea sensor were examined. Response characteristics for the determination of urea in synthetic physiological saline solution (pH 7.4) were as follows. That is, the concentration range of linear response, slope of linear response (sensitivity), and response time were 5.0 × 10-4 ∼ 5.0 × 10-2M, 31.6 mV/decade and 4∼10 min, respectively.
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